Journal: Membranes

Article Title: Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies

doi: 10.3390/membranes13050511

Figure Lengend Snippet: Chromatography procedure for bind and elute studies on Protein A stationary phases.

Article Snippet: Polyclonal human immunoglobulin (hIgG) was purchased from Lee BioSolutions (Maryland Heights, MO, USA).

Techniques: Chromatography

Journal: Membranes

Article Title: Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies

doi: 10.3390/membranes13050511

Figure Lengend Snippet: Normalized breakthrough curves from loading hIgG at 5 mg/mL onto Protein A membranes. Flow rate was varied from 5 to 120 s residence time for all stationary phases. ( A ) Cytiva HiTrap Fibro™ PrismA, ( B ) Purilogics Purexa™ PrA, ( C ) Gore ® Protein Capture Device, ( D ) Sartorius Sartobind ® Protein A, ( E ) summary of measured DBC 10 for all stationary phases. (The dotted lines are guides to the eye).

Article Snippet: Polyclonal human immunoglobulin (hIgG) was purchased from Lee BioSolutions (Maryland Heights, MO, USA).

Techniques:

Journal: Membranes

Article Title: Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies

doi: 10.3390/membranes13050511

Figure Lengend Snippet: Equilibrium binding capacity (EBC) measurements at 120 s residence time. All membranes were loaded at 5 mg/mL hIgG in 1X PBS pH 7.4 buffer and eluted using 0.1 M citric acid pH 3 buffer.

Article Snippet: Polyclonal human immunoglobulin (hIgG) was purchased from Lee BioSolutions (Maryland Heights, MO, USA).

Techniques: Binding Assay

Journal: Membranes

Article Title: Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies

doi: 10.3390/membranes13050511

Figure Lengend Snippet: Elution behavior for Protein A membrane adsorbers at 12 and 120 s residence time. Membranes were saturated using 2 mg/mL hIgG in 1X PBS pH 7.4 buffer and eluted using 0.1 M citric acid pH 3 buffer. ( A ) Cytiva HiTrap Fibro™ PrismA, ( B ) Purilogics Purexa™ PrA, ( C ) Gore ® Protein Capture Device, ( D ) Sartorius Sartobind ® Protein A, ( E ) summary of measured EVs for all stationary phases at both flow rates, with the membrane to dead volume ratio graphed on the secondary y -axis.

Article Snippet: Polyclonal human immunoglobulin (hIgG) was purchased from Lee BioSolutions (Maryland Heights, MO, USA).

Techniques: Membrane

Journal: Membranes

Article Title: Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies

doi: 10.3390/membranes13050511

Figure Lengend Snippet: Dynamic binding capacity (DBC 10 ) versus run number for three Protein A membrane adsorbers: Cytiva HiTrap Fibro™ PrismA, Purilogics Purexa™ PrA, and Gore ® Protein Capture Device. Two CVs of 0.1 M sodium hydroxide were used for CIP procedure after each run. All membrane adsorbers were loaded at 30 s residence time using 5 mg/mL hIgG in 1X PBS pH 7.4 buffer.

Article Snippet: Polyclonal human immunoglobulin (hIgG) was purchased from Lee BioSolutions (Maryland Heights, MO, USA).

Techniques: Binding Assay, Membrane